Adrenal Fatigue Test

Adrenal Fatigue Tests


When Adrenal Fatigue was first discovered many of today’s tests did not exist. A diagnosis was made on the basis of the patient’s symptoms alone or together with a series of very useful physical tests. One of these physical tests is described below in the next paragraph. Naturally these physical tests are clearly much more subjective than traditional blood, saliva and urine tests. But they can be a useful diagnostic tool, especially if positive results from these physical tests are accompanied by other positive test results from modern laboratory tests. In addition, several websites offer Adrenal Fatigue Questionnaires or Adrenal Fatigue Quizzes. These can be a very handy tool to supplement other Adrenal Fatigue Tests and help diagnose Adrenal Fatigue. Answers to the questions will create a picture of how functional or dysfunctional your adrenal glands are. We have a short Adrenal Fatigue Quiz here.


In 1929, Dr. Arroyo discovered a useful test to detect Adrenal Fatigue: the Iris Contraction Test” This Adrenal Fatigue test goes as follows: Darken the room and shine a flashlight across one eye (not directly into the eye) and watch what happens in the mirror. Under normal circumstances the pupil will immediately contract and will stay contracted. But if you have poor adrenal function the pupil can’t hold the contraction and will, either waver between contracted and dilated, or will initially contract but then dilate within 2 minutes (despite the light shining on it) and the dilation will last for 30-45 seconds before it contracts again. You can retest this monthly as an indicator of recovery (as you recover from Adrenal Fatigue the pupil will hold the contraction longer). The inability of the pupil to remain contracted is more typically present in moderate to severe cases and usually not present in milder cases of Adrenal Fatigue (1).


Cortisol Tests


The lab tests that most conventional doctors use only measure extreme adrenal dysfunction (Cushing’s syndrome and Addison’s disease) and those tests also don’t take into account the natural changes of adrenal function throughout the day (diurnal rhythm). The best way to determine adrenal function is to test cortisol at a variety of points through the day to see if cortisol is spiking and dropping at the correct times.


Circadian Release Cortisol


24-hour Saliva hormone testing (rather than blood or urine testing) is considered to be the best lab test for detecting Adrenal Fatigue because:



Salivary cortisol testing may not be readily accepted by mainstream medicine, there are a lot of scientific studies published in peer-reviewed journals demonstrating its utility clinically (2),(3),(4),(5).


How Does The Salivary Cortisol Collection Process Work?


Saliva testing is an easy and noninvasive way of assessing the active hormones available for use by your body. Typically your doctor or you (in the case of self testing through a direct-consumer-lab) are to collect 4 separate saliva specimens over the course of a single day into a sterile container (to be provided by your healthcare professional or direct-to-consumer lab). Each collection will take approximately 10 to 15 minutes. You should avoid coffee, caffeinated beverages and food until after the saliva collection. Exercise should also be avoided. The specimens should be stored in the refrigerator until all have been completed and subsequently the specimens should be mailed to the (direct-to-consumer) lab for analysis. Usually the results will return in approximately 2 weeks and will be discussed at your next appointment or will be emailed to you.


Where To Take A Salivary Cortisol Test?


You should discuss with your doctor or healthcare professional about your specific situation and symptoms and see if a salivary cortisol test would be useful for you. Unfortunately a lot of doctors and healthcare professionals are still unaware of the validity, reliability of salivary hormone testing. In these situations direct-to-consumer labs, such as True Health Labs, are a good alternative also for those who are uninsured, have a doctor who is not willing to order lab tests due to lack of awareness or who is not willing to be comprehensive. These lab tests allow patients to order tests directly or work with a practitioner for testing.


You can also purchase the below selected Adrenal Function tests directly via Amazon:


Diurnal Cortisol (Cx4) – 4 Panel (Full Day) Cortisol Hormone Stress Level Imbalance Test Kit


Female/Male Saliva Profile I – Test Kit For 5 Hormone Level Imbalances (E2, Pg, T, DS and C)


Below are, in NO special order, some lab facilities that provide Adrenal Fatigue Tests. The first two below are for the United States (except for NY, NJ, RI, MA or MD), then you’ll see other countries below that. All of these can be used without a prescription (unless stated otherwise): 



True Health Labs was created to make lab testing directly available to those who are uninsured, underinsured, whose doctor refuses to order tests, and those who simply want to make their own healthcare choices. is a Direct Access Testing (DAT) company that provides lab tests from dozens of CLIA certified laboratories both in the USA and internationally. Click here.



My Med LabFrom the beginning, the mission of MyMedLab has been simple; to provide consumers with an affordable and convenient way to privately learn about their health. The MyMedLab process combines transparent pricing of state-of-the-art testing with the services of a board certified physician in every state. This unique combination eliminates the cost and inconvenience of a doctor’s visit just to order routine testing. It provides essential Physician oversight throughout the entire process while still guaranteeing complete privacy. MyMedLab results never become part of any permanent medical record unless you provide them.



  1. Ask your local healthcare professional to order a saliva cortisol test kit from Rocky Mountain Labs in Canada
  2. is able to ship saliva cortisol test kits to Canada


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Adrenal Fatigue Information & Recovery Package - Only $ 19
Written in a clear, concise and easy-to-understand manner. The Adrenal Fatigue Information & Recovery Package is a very complete information package designed to give you a headstart in your recovery from Adrenal Fatigue. You will receive 1 Adrenal Health Inventory Questionnaire and 1 Digital Adrenal Fatigue Recovery Workbook that contains Self Assessments, Exercises, Educational Backgrounds and a step-by-step approach to tackle Adrenal Fatigue.
Price: $19.00

Digital Download Only! All materials are DIGITAL and sent to you INSTANTLY in PDF format compressed in a .zip file. Nothing will be physically mailed to you.  Download to any computer or iPad, just NOT a phone. Instant download means you can START TODAY!


References & Further Reading


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